吴炳耀1，李敏2，于金兰3，刘国礼1，叶松1，孙立新4

    1.安徽理工大学医学院，安徽淮南 232001；2.南通市妇幼保健院；

    3.南京空军机关医院；4.江苏出入境检验检疫局

   摘要：目的了解江苏地区尖音库蚊复合组种群构成，为进一步防控蚊虫蚊媒病奠定基础。方法于2012年采集江苏省连云港、常熟、张家港、盐城、泰州、徐州、南京、南通和无锡9个地区蚊虫，通过扩增mtDNA-COI基因、测序和基因比对，对上述地区尖音库蚊复合组进行分析。结果mtDNA-COI基因分析显示，江苏地区的尖音库蚊复合组差异明显，进化树显示分为2支，南京、泰州、无锡、盐城、徐州地区为一支，与淡色库蚊同源性较高；连云港、南通、常熟、张家港地区为1支，与致倦库蚊同源性较高。结论江苏地区尖音库蚊复合组存在淡色库蚊和致倦库蚊两类基因型，未发现尖音库蚊和骚扰库蚊基因型，亦未发现新的隐藏种。

    关键词：尖音库蚊复合组；线粒体DNA细胞色素氧化酶亚基；种群组成；复合组；调查

    中图分类号：R372；R184.31　文献标识码：B

    Analysis on the population of Culexpipiens complexgroup in Jiangsu area

    WU Bing-yao*, LI Min, LIU Guo-li, YE Song, SUNLi-xin

    *Department of Medicine, Anhui University of Scienceand Technology, Huainan, 'Anhui 232001, China

    Abstract:   Objective   To understand thepopulation of Culexpipiens complex in Jiangsu for the furtherprevention and control of the mosquitoes and mosquito-bornediseases.   Methods    The mosquitoes were collectedin Jiangsu province,  (Lianyungang,Zhangjiagang, Yancheng,Taizhou, Changshu, Xuzhou, Nanjing, Nantong and Wuxi) in 2012. Thepopulations of Culexpipiens complex from above areas were analyzedthrough the amplification of mtDNA-COI gene, gene sequencing andcomparison.   Results    The analysis ofCulexpipiens complex mtDNA-COI gene in Jiangsu area shows, thedifferences of Culexpipiens complex in Jiangsu changessignificantly ,and the phylogenetic tree divided into two branches,with Nanjing、Taizhou、Wuxi、Yancheng、Xuzhou area as a branch, wheremosquitoes have high homology with Culexpipienspallens, the otherbranch include Lianyungang、Nantong,、Changshu、Zhangjiagang area,where mosquitoes have homology with Culexpipiensquinquefasciatus.  Conclusion    The mtDNA-COI genes of Culexpipienscomplex in Jiangsu have two genotypes, similar withCulexpipienspallens and Culexpipiensquinquefasciatus. This studyfound that there is no Culexpipienspipiens、Culexpipiensmolestus andcryptic species of Culexpipiens complex in Jiangsu.

    Key words:   Culexpipiens complex；MtDNA-COI；Population； Complex group；Investigate

    《中国国境卫生检疫杂志》2013年10月刊